Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human 5HT1B Receptor protein to be purified and analysed in vitro.
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human 5HT1B Receptor protein has a MW of 43.6 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Nanodisc Human 5HT1B Receptor Protein (A325074)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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