Synthetic Membrane Nanoparticle Human Transmembrane 4 L6 Family Member 1 Protein
Description
Full-length transmembrane human Transmembrane 4 L6 Family Member 1 protein expressed in membrane nanoparticles.
Applications
ELISA, FACS
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human Transmembrane 4 L6 Family Member 1 protein has a MW of 21.6 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Membrane Nanoparticle Human Transmembrane 4 L6 Family Member 1 Protein (A318477)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Membrane Nanoparticle Human Transmembrane 4 L6 Family Member 1 Protein (A318477). Serial diluted Anti-Transmembrane 4 L6 Family Member 1 Humanized Antibody [AGX101 Biosimilar] - Azide free (A318815) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-Transmembrane 4 L6 Family Member 1 Humanized Antibody [AGX101 Biosimilar] - Azide free (A318815) binding with TM4SF1 full length membrane nanoparticles is 4.174 µg/ml.
Figure 2: Synthetic Membrane Nanoparticle Human Transmembrane 4 L6 Family Member 1 Protein (A318477)
FACS analysis of Synthetic Membrane Nanoparticle Human Transmembrane 4 L6 Family Member 1 Protein (A318477): A. Negative Control 1: Synthetic Membrane Nanoparticle Human Transmembrane 4 L6 Family Member 1 Protein (A318477) samples were stained only with Goat Anti-Human lgG Antibody (Alexa Fluor 488). B. Negative Control 2: Control membrane nanoparticles samples were stained with Anti-Transmembrane 4 L6 Family Member 1 Humanized Antibody [AGX101 Biosimilar] - Azide free (A318815) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). C. Negative Control 3: Synthetic Membrane Nanoparticle Human Transmembrane 4 L6 Family Member 1 Protein (A318477) samples were stained with Anti-CCR8 Antibody (an irrelevant antibody) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). D. Synthetic Membrane Nanoparticle Human Transmembrane 4 L6 Family Member 1 Protein (A318477) samples were stained with Anti-Transmembrane 4 L6 Family Member 1 Humanized Antibody [AGX101 Biosimilar] - Azide free (A318815) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488).
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