Synthetic Membrane Nanoparticle Human CD20 Protein (A318472)

ELISA plates were pre-coated with 0.5 µg/per well Synthetic Membrane Nanoparticle Human CD20 Protein (A318472). Serial diluted Anti-CD20 Humanized Antibody [Obinutuzumab Biosimilar] - Azide free (A318814) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-CD20 Humanized Antibody [Obinutuzumab Biosimilar] - Azide free (A318814) binding with CD20 full length membrane nanoparticles is 128.8 µg/ml.

FACS analysis of Synthetic Membrane Nanoparticle Human CD20 Protein (A318472): A. Negative Control 1: Synthetic Membrane Nanoparticle Human CD20 Protein (A318472) samples were stained only with Goat Anti-Human lgG Antibody (Alexa Fluor 488). B. Negative Control 2: Control membrane nanoparticles samples were stained with Anti-CD20 Humanized Antibody [Obinutuzumab Biosimilar] - Azide free (A318814) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). C. Negative Control 3: Synthetic Membrane Nanoparticle Human CD20 Protein (A318472) samples were stained with Anti-CCR8 Antibody (an irrelevant antibody) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). D. Synthetic Membrane Nanoparticle Human CD20 Protein (A318472) samples were stained with Anti-CD20 Humanized Antibody [Obinutuzumab Biosimilar] - Azide free (A318814) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488).