Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481)

ELISA plates were pre-coated with 0.5 µg/per well Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481). Serial diluted Anti-CCR4 Humanized Antibody [Mogamulizumab Biosimilar] - Azide free (A318876) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-CCR4 Humanized Antibody [Mogamulizumab Biosimilar] - Azide free (A318876) binding with CCR4 full length membrane nanoparticles is 308.3 µg/ml.

FACS analysis of Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481): A. Negative Control 1: Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481) samples were stained only with Goat Anti-Human lgG Antibody (Alexa Fluor 488). B. Negative Control 2: Control membrane nanoparticles samples were stained with Anti-CCR4 Humanized Antibody [Mogamulizumab Biosimilar] - Azide free (A318876) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). C. Negative Control 3: Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481) samples were stained with Anti-CCR8 Antibody (an irrelevant antibody) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). D. Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481) samples were stained with Anti-CCR4 Humanized Antibody [Mogamulizumab Biosimilar] - Azide free (A318876) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488).