Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein
Description
Full-length transmembrane human Cannabinoid Receptor I protein expressed on the cell surface of membrane nanoparticles.
Applications
ELISA, FACS
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human Cannabinoid Receptor I protein has a MW of 52.7 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488). Serial diluted Anti-Cannabinoid Receptor I Antibody [DM144] - Azide free (A318564) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-Cannabinoid Receptor I Antibody [DM144] - Azide free (A318564) binding with CB1 full length membrane nanoparticles is 439.6 µg/ml.
Figure 2: Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488)
FACS analysis of Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488): A. Negative Control 1: Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488) samples were stained only with Goat Anti-Human lgG Antibody (Alexa Fluor 488). B. Negative Control 2: Control membrane nanoparticles samples were stained with Anti-Cannabinoid Receptor I Humanized Antibody [Nimacimab Biosimilar] - Azide free (A318908) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). C. Negative Control 3: Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488) samples were stained with Anti-CCR8 Antibody (an irrelevant antibody) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). D. Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488) samples were stained with Anti-Cannabinoid Receptor I Humanized Antibody [Nimacimab Biosimilar] - Azide free (A318908) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488).
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