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Anti-SYT1 Antibody (A83675)

Western Blot - Anti-SYT1 Antibody (A83675) - Antibodies.com Immunohistochemistry - Anti-SYT1 Antibody (A83675) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to SYT1 for ELISA, WB and IHC.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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+1 (314) 370-6046
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Name
Anti-SYT1 Antibody
Description
Goat polyclonal antibody to SYT1.
Applications
ELISA, WB, IHC
Dilutions
ELISA: 1:2,000, WB: 0.3-1µg/ml, IHC: 4-6µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region of human SYT1.
Sequence
C-RDLQSAEKEEQEK
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
65 kDa
Predicted MW
47.6kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-SYT1 Antibody (A83675) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-SYT1 Antibody (A83675)
SYT1 expression in Human Brain (Hippocampus) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-SYT1 Antibody (A83675) at 1µg/ml and detected by chemiluminescence.
Immunohistochemistry - Anti-SYT1 Antibody (A83675) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-SYT1 Antibody (A83675)
SYT1 expression in Human Brain analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-SYT1 Antibody (A83675) at 3.8µg/ml and revealed with alkaline phosphatase (AP).
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