The antibody detects endogenous level of total Synaptotagmin 1 / 2 protein.
Applications
WB, IF
Dilutions
Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around aa.200~204 / 197~201 (R-K-T-L-N) derived from Human Synaptotagmin 1 / 2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1.0mg / ml
Predicted MW
46kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
DKFZp781D2042, FLJ42519, p65, SVP65, Synaptotagmin 2, Synaptotagmin I, Synaptotagmin II, Synaptotagmin-1, SYT, Syt1, SYT1_HUMAN, Syt2, SytI
Western blot analysis of extracts from Hela cells using Synaptotagmin 1/2 (Ab-202/199) Antibody #21293 and the same antibody preincubated with blocking peptide.
