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Anti-SUZ12 Antibody (A12169)

Western Blot - Anti-SUZ12 Antibody (A12169) - Antibodies.com Western Blot - Anti-SUZ12 Antibody (A12169) - Antibodies.com Immunofluorescence - Anti-SUZ12 Antibody (A12169) - Antibodies.com Western Blot - Anti-SUZ12 Antibody (A12169) - Antibodies.com
Knockout Validated
$470
Product Datasheet

Rabbit polyclonal antibody to SUZ12 for WB, ICC/IF and IP.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-SUZ12 Antibody
Description
Rabbit polyclonal antibody to SUZ12.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:100, IP: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2).
Sequence
CHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
83 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ChET 9 protein, CHET9, Chromatin precipitated E2F target 9 protein, JJAZ1, Joined to JAZF1 protein, KIAA0160, Polycomb protein SUZ12, Suppressor of zeste 12 homolog, Suppressor of zeste 12 protein homolog, SUZ12 polycomb repressive complex 2 subunit, SUZ12_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-SUZ12 Antibody (A12169) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-SUZ12 Antibody (A12169)
Western blot analysis of extracts of various cell lines, using Anti-SUZ12 Antibody (A12169) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-SUZ12 Antibody (A12169) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-SUZ12 Antibody (A12169)
Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using Anti-SUZ12 Antibody (A12169) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-SUZ12 Antibody (A12169) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-SUZ12 Antibody (A12169)
Immunofluorescence analysis of C6 cells using Anti-SUZ12 Antibody (A12169) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-SUZ12 Antibody (A12169) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Anti-SUZ12 Antibody (A12169)
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-SUZ12 Antibody (A12169). This Western blot was performed on the immunoprecipitate using Anti-SUZ12 Antibody (A12169) at a dilution of 1:500.
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