Principle of Assay
This kit provides a simple and easy assay for the quantitative determination of superoxide dismutase (SOD) enzyme activity in serum, plasma, animal and plant tissues, cells lysates, and other biological fluids. In the assay, superoxide anion (O2-) is generated by a xanthine oxidase catalyzed reaction. O2- reacts with a tetrazolium salt (WST-8) dye to form a water-soluble colored formazan product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. This inhibitory activity of SOD is measured by colorimetric method at OD 450 nm. The activity of SOD is negatively correlated with the amount of formazan produced.
