Principle of Assay
The superoxide anion in plant, animal tissues, serum and other samples reacts with hydroxylamine hydrochloride to produce NO2- , which then reacts with Gris reagent to generate a red azo compound with a characteristic absorption peak at 540 nm. The O2- content in the sample can be calculated based on the A540 value. The kit can detect samples of plants, animal tissues, serum, and cells.
