Anti-Sumo 1 Antibody (A307946)

Name: Anti-Sumo 1 Antibody
Brand: Antibodies.com
SKU: A307946
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to Sumo 1 for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-Sumo 1 Antibody

Description

Rabbit polyclonal antibody to Sumo 1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 40-100 of human SUMO1 (NP_003343.1).

Sequence

MTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHST

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

16 kDa / 80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DAP1, GAP modifying protein 1, GAP-modifying protein 1, GMP 1, GMP1, OFC10, PIC 1, PIC1, SENP2, Sentrin, Sentrin 1, Small ubiquitin related modifier 1, Small ubiquitin-like modifier 1, Small ubiquitin-related modifier 1, SMT3, SMT3 homolog 3, SMT3 suppressor of mif two 3 homolog 1, SMT3, yeast, homolog 3, Smt3C, SMT3H3, SUMO-1, SUMO1, SUMO1_HUMAN, Ubiquitin homology domain protein PIC1, Ubiquitin Like 1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, UBL 1, UBL1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Sumo 1 Antibody (A307946)

Western blot analysis of extracts from wild type(WT) and SUMO1 Rabbit polyclonal antibody knockout (KO) 293T cells, using Anti-Sumo 1 Antibody (A307946) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Sumo 1 Antibody (A307946)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Sumo 1 Antibody (A307946) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Sumo 1 Antibody (A307946)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Sumo 1 Antibody (A307946) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Sumo 1 Antibody (A307946)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Sumo 1 Antibody (A307946) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.