Western Blot - Anti-Substance P Antibody (A307462)
Western blot analysis of extracts from normal (control) and Substance P knockout (KO) 293T cells, using Anti-Substance P Antibody (A307462) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 300s.
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