StreptaClick® Antibody Labeling and Biotinylation Kit - 4 Color (A329038)

StreptaClick® Antibody Labeling and Biotinylation Kit - 4 Color

This kit uses click chemistry to conjugate four fluorochromes to primary antibodies after biotinylation.

IHC, ICC/IF

StreptaClick® Antibody Labeling and Biotinylation Kits provide a rapid, robust, and flexible method to conjugate fluorochromes to antibodies after biotinylation using click chemistry. Once labeled with a fluorochrome, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. StreptaClick® labeling occurs within 5 minutes, is highly efficient and can be carried out at any volume, concentration, or buffer. The antibody-fluorochrome conjugates are then mixed together in a single tube with the Biotin Block Buffer provided, after which they are ready to be used as primary antibodies in a normal IHC or flow cytometry workflow. StreptaClick®-antibody conjugates are compatible with DAPI, other directly conjugated primary antibodies and conventional secondary antibodies in the same assay. StreptaClick® is not compatible with other streptavidin-based techniques.

Liquid and Resin

The StreptaClick® antibody labeling reagent contains 0.09% Sodium Azide.

Shipped at +4°C. Upon delivery, open immediately and store specific components at -20°C and others at +4°C according to product manual. Avoid exposure to light!

The StreptaClick® Antibody 4 Color Labeling Kit contains the labeling reagents for conjugating antibodies with AZDye™ 488 Atto™ 542, AZDye™ 594, AZDye™ 647. Each color contains 500µl of StreptaClick® antibody labeling reagent, capable of labeling 50µg of biotinylated antibody, sufficient for approximately 50 slides.