Rabbit polyclonal antibody to STAT5 (phospho Tyr694).
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:100-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around Y694 of human STAT5 (NP_003143.2/NP_036580.2).
Sequence
DGYVK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Molecular Weight
90 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
MGF, Signal transducer and activator of transcription 5A, Signal Transducer and Activator of Transcription 5B, STA5A_HUMAN, STAT 5A, STAT 5B, STAT5A, STAT5B, Transcription factor STAT5A, Transcription factor STAT5B
Figure 1: Western Blot - Anti-STAT5 (phospho Tyr694) Antibody (A91399)
Western blot analysis of extracts of K562 cells, using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at 1:1,000 dilution. K-562 cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Western Blot - Anti-STAT5 (phospho Tyr694) Antibody (A91399)
Western blot analysis of extracts of A-431 cells, using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at 1:1,000 dilution or STAT5B antibody (A12356). A-431 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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