Anti-STAT4 Antibody [ARC1071] (A306876)

Name: Anti-STAT4 Antibody [ARC1071]
Brand: Antibodies.com
SKU: A306876
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1071] antibody to STAT4 for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-STAT4 Antibody [ARC1071]

Description

Rabbit monoclonal [ARC1071] antibody to STAT4.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 649-748 of human STAT4 (Q14765).

Sequence

MAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1071

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

86 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Signal transducer and activator of transcription 4, SLEB11, STAT4_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-STAT4 Antibody [ARC1071] (A306876)

Western blot analysis of extracts of various cell lines, using Anti-STAT4 Antibody [ARC1071] (A306876) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunofluorescence - Anti-STAT4 Antibody [ARC1071] (A306876)

Immunofluorescence analysis of C6 cells using Anti-STAT4 Antibody [ARC1071] (A306876) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-STAT4 Antibody [ARC1071] (A306876)

Immunofluorescence analysis of NIH-3T3 cells using Anti-STAT4 Antibody [ARC1071] (A306876) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-STAT4 Antibody [ARC1071] (A306876)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-STAT4 Antibody [ARC1071] (A306876). This Western blot was performed on the immunoprecipitate using Anti-STAT4 Antibody [ARC1071] (A306876) at a dilution of 1:1000.

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