Anti-STAT3 Antibody [ARC2603] (A308912)

Name: Anti-STAT3 Antibody [ARC2603]
Brand: Antibodies.com
SKU: A308912
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2603] antibody to STAT3 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-STAT3 Antibody [ARC2603]

Description

Rabbit monoclonal [ARC2603] antibody to STAT3.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human STAT3 (P40763).

Sequence

TKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2603

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

86 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3, Signal transducer and activator of transcription 3 (acute phase response factor), STAT 3, STAT3_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-STAT3 Antibody [ARC2603] (A308912)

Western blot analysis of various lysates, using Anti-STAT3 Antibody [ARC2603] (A308912) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-STAT3 Antibody [ARC2603] (A308912)

Western blot analysis of extracts from normal (control) and STAT3 knockout (KO) HeLa(KO) cells, using Anti-STAT3 Antibody [ARC2603] (A308912) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-STAT3 Antibody [ARC2603] (A308912)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-STAT3 Antibody [ARC2603] (A308912) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-STAT3 Antibody [ARC2603] (A308912)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-STAT3 Antibody [ARC2603] (A308912) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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