Anti-STAT3 Antibody (A83190)

Name: Anti-STAT3 Antibody
Brand: Antibodies.com
SKU: A83190
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to STAT3 for ELISA, IHC, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-STAT3 Antibody

Description

Goat polyclonal antibody to STAT3.

Applications

ELISA, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:8,000, WB: 2µg/ml, IHC: 4-6µg/ml, IF: 10µg/ml

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to the C terminal region of human STAT3 (Isoform 1 and 2).

Sequence

DMELTSECATSPM

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

80-85 kDa

Predicted MW

80kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3, Signal transducer and activator of transcription 3 (acute phase response factor), STAT 3, STAT3_HUMAN

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Immunohistochemistry - Anti-STAT3 Antibody (A83190)

STAT3 expression in Mouse Thymus analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-STAT3 Antibody (A83190) at 4µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 2: Immunohistochemistry - Anti-STAT3 Antibody (A83190)

Negative control for STAT3 expression in Mouse Thymus analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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Figure 3: Immunofluorescence - Anti-STAT3 Antibody (A83190)

STAT3 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-STAT3 Antibody (A83190) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and weak nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-STAT3 Antibody (A83190)

STAT3 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-STAT3 Antibody (A83190) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.