Anti-Src Antibody [ARC0378] (A307395)

Name: Anti-Src Antibody [ARC0378]
Brand: Antibodies.com
SKU: A307395
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0378] antibody to Src for WB, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-Src Antibody [ARC0378]

Description

Rabbit monoclonal [ARC0378] antibody to Src.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Src (P12931).

Sequence

MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0378

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ASV, Avian sarcoma virus, c SRC, CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA, cSrc, EC 2.7.10.2, Neuronal CSRC tyrosine specific protein kinase, Neuronal SRC, Oncogene SRC, OTTHUMP00000174476, OTTHUMP00000174477, p60 Src, p60-Src, p60Src, pp60c src, pp60c-src, pp60csrc, Proto oncogene tyrosine protein kinase Src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, Protooncogene SRC, Protooncogene SRC Rous sarcoma, SRC Oncogene, SRC proto oncogene non receptor tyrosine kinase, SRC1, SRC_HUMAN, Tyrosine kinase pp60c src, Tyrosine protein kinase SRC 1, Tyrosine protein kinase SRC1, v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog, V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian), v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Src Antibody [ARC0378] (A307395)

Western blot analysis of extracts of various cell lines, using Anti-Src Antibody [ARC0378] (A307395) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Src Antibody [ARC0378] (A307395)

Western blot analysis of extracts from wild type (WT) and Src knockout (KO) HeLa cells, using Anti-Src Antibody [ARC0378] (A307395) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 3: Western Blot - Anti-Src Antibody [ARC0378] (A307395)

Western blot analysis of extracts of Jurkat cells, using Anti-Src Antibody [ARC0378] (A307395) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 4: Immunofluorescence - Anti-Src Antibody [ARC0378] (A307395)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Src Antibody [ARC0378] (A307395) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).