Western blot analysis of extracts of various cell lines, using Anti-SQSTM1/p62 Antibody (A80499) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-SQSTM1/p62 Antibody (A80499) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-SQSTM1/p62 Antibody (A80499) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-SQSTM1/p62 Antibody (A80499) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using Anti-SQSTM1/p62 Antibody (A80499) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 100µg extracts of HepG2 cells using 3µg of Anti-SQSTM1/p62 Antibody (A80499). This Western blot was performed on the immunoprecipitate using Anti-SQSTM1/p62 Antibody (A80499) at a dilution of 1:1000.
