SPRY1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-SPRY1 Antibody (A83695) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic and Golgi apparatus staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.
Figure 2: Western Blot - Anti-SPRY1 Antibody (A83695)
SPRY1 expression in Human Kidney lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SPRY1 Antibody (A83695) at 0.3µg/ml and detected by chemiluminescence.
SPRY1 expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-SPRY1 Antibody (A83695) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic and Golgi apparatus staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.
SPRY1 expression in HEK293 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-SPRY1 Antibody (A83695) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 0.4µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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