Anti-SP1 (phospho Thr453) Antibody (A93778)

Name: Anti-SP1 (phospho Thr453) Antibody
Brand: Antibodies.com
SKU: A93778
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to SP1 (phospho Thr453) for WB, IHC, IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-SP1 (phospho Thr453) Antibody

Description

Rabbit polyclonal antibody to SP1 (phospho Thr453).

Specificity

This antibody detects endogenous levels of SP1 only when phosphorylated at Thr453.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:1000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human SP1 around the phosphorylation site of Thr453 (amino acids 421-470).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

80kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

SP 1, Sp1 transcription factor, SP1_HUMAN, Specificity protein 1, Transcription factor Sp1, TSFP 1, TSFP1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-SP1 (phospho Thr453) Antibody (A93778)

Western blot analysis of lysates from A549 cells using Anti-SP1 (phospho Thr453) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunofluorescence - Anti-SP1 (phospho Thr453) Antibody (A93778)

Immunofluorescence analysis of HeLa cells using Anti-SP1 (phospho Thr453) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Immunohistochemistry - Anti-SP1 (phospho Thr453) Antibody (A93778)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-SP1 (phospho Thr453) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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