Anti-SOX10 Antibody [SOX10/992] (A250014)

Name: Anti-SOX10 Antibody [SOX10/992]
Brand: Antibodies.com
SKU: A250014
Price: 525.0 USD
Availability: InStock

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$525

Mouse monoclonal [SOX10/992] antibody to SOX10 for ELISA and WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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Tags:

Monospecific Antibody Neuroepithelial Marker Oligodendrocyte Marker Schwann Cell Marker

Specifications

Name

Anti-SOX10 Antibody [SOX10/992]

Description

Mouse monoclonal [SOX10/992] antibody to SOX10.

Specificity

The specificity of this monoclonal antibody to its intended target was validated by HuProt^TM Array, containing more than 19,000 full-length human proteins. Recognizes a protein of ~55kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Applications

ELISA, WB

Dilutions

WB: 1-2 µg/ml

Reactivity

Human

Immunogen

Recombinant fragment, around amino acids 115-269, of human SOX10 protein. The exact sequence is proprietary.

Host

Mouse

Clonality

Monoclonal

Clone ID

SOX10/992

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein A/G chromatography.

Concentration

200 µg/ml

Molecular Weight

49-58 kDa

Product Form

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-SOX10 Antibody [SOX10/992] - BSA and Azide free (A253194).

Synonyms

DOM, Dominant megacolon mouse human homolog of, MGC15649, PCWH, SOX 10, SOX10_HUMAN, SRY (sex determining region Y) box 10, SRY box 10, SRY box containing gene 10, SRY related HMG box gene 10, Transcription factor SOX 10, Transcription factor SOX-10, WS2E, WS4, WS4C

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SOX10 Antibody [SOX10/992] (A250014)

Western blot analysis of (A) recombinant SOX10 protein and (B) A375 cell lysate using Anti-SOX10 Antibody [SOX10/992].

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Figure 2: SDS-PAGE - Anti-SOX10 Antibody [SOX10/992] (A250014)

SDS-PAGE analysis of Anti-SOX10 Antibody [SOX10/992] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Figure 3: Anti-SOX10 Antibody [SOX10/992] (A250014)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-SOX10 Antibody [SOX10/992]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.