Anti-Sonic Hedgehog Antibody (A16913)

Name: Anti-Sonic Hedgehog Antibody
Brand: Antibodies.com
SKU: A16913
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Sonic Hedgehog for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Sonic Hedgehog Antibody

Description

Rabbit polyclonal antibody to Sonic Hedgehog.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Sonic Hedgehog (Shh) (NP_000184.1).

Sequence

PGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDDGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSATGEPEASSGSGPPSGGALG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

19 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

HHG 1, HHG-1, HHG1, HLP 3, HLP3, Holoprosencephaly 3, HPE 3, HPE3, MCOPCB5, shh, SHH_HUMAN, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, Sonic hedgehog homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog protein, Sonic hedgehog protein C-product, TPT, TPTPS

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Sonic Hedgehog Antibody (A16913)

Western blot analysis of extracts of various cell lines, using Anti-Sonic Hedgehog Antibody (A16913) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-Sonic Hedgehog Antibody (A16913)

Western blot analysis of extracts of Rat lung, using Anti-Sonic Hedgehog Antibody (A16913) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-Sonic Hedgehog Antibody (A16913)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Sonic Hedgehog Antibody (A16913) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Sonic Hedgehog Antibody (A16913)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Sonic Hedgehog Antibody (A16913) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.