Western Blot - Anti-Sonic Hedgehog Antibody (A16913)
Western blot analysis of extracts of various cell lines, using Anti-Sonic Hedgehog Antibody (A16913) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-Sonic Hedgehog Antibody (A16913)
Western blot analysis of extracts of Rat lung, using Anti-Sonic Hedgehog Antibody (A16913) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Sonic Hedgehog Antibody (A16913) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Sonic Hedgehog Antibody (A16913) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using Anti-Sonic Hedgehog Antibody (A16913) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of rat liver cells using Anti-Sonic Hedgehog Antibody (A16913) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
