Figure 1: Western Blot - Anti-SOD2/MnSOD Antibody (A83715)
SOD2/MnSOD expression in Human Cerebellum (A), Mouse Brain (B), Rat Brain (C), and Pig Brain (D) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SOD2/MnSOD Antibody (A83715) at 0.3µg/ml and detected by chemiluminescence.
Figure 2: Western Blot - Anti-SOD2/MnSOD Antibody (A83715)
SOD2/MnSOD expression in HeLa, HepG2, HEK293 and NIH3T3 analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SOD2/MnSOD Antibody (A83715) at 0.1µg/ml and detected by chemiluminescence.
Figure 3: Western Blot - Anti-SOD2/MnSOD Antibody (A83715)
SOD2/MnSOD expression in Mouse (A) and Rat (B) Spinal Cord lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SOD2/MnSOD Antibody (A83715) at 0.01µg/ml and detected by chemiluminescence.
SOD2/MnSOD expression in Human Skeletal Muscle analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-SOD2/MnSOD Antibody (A83715) at 5µg/ml and revealed with alkaline phosphatase (AP).
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