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Anti-Superoxide Dismutase 1 Antibody (A84197)

This product is identical to Novus (NB100-60944). Discover more for less.

Western Blot - Anti-Superoxide Dismutase 1 Antibody (A84197) - Antibodies.com Western Blot - Anti-Superoxide Dismutase 1 Antibody (A84197) - Antibodies.com Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A84197) - Antibodies.com Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A84197) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to Superoxide Dismutase 1 for ELISA, WB and IF.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
3-5 Business Days

Telephone
+1 (314) 370-6046
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Name
Anti-Superoxide Dismutase 1 Antibody
Description
Goat polyclonal antibody to Superoxide Dismutase 1.
Applications
ELISA, WB, IF
Dilutions
ELISA: 1:8,000, WB: 0.01-0.03µg/ml, IF: 10µg/ml
Reactivity
Human, Mouse
Immunogen
Synthetic peptide corresponding to the internal region of human Superoxide Dismutase 1.
Sequence
C-SRKHGGPKDEERH
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
18 kDa
Predicted MW
15.9kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
ALS, ALS1, Amyotrophic Lateral Sclerosis 1 (Adult), Amyotrophic lateral sclerosis 1 adult, Cu,Zn Superoxide Dismutase, EC 1.15.1.111, Cu/Zn SOD, Cu/Zn superoxide dismutase, Epididymis secretory protein Li 44, HEL S 44, Homodimer, hSod1, Indophenoloxidase A, IPOA, Mn superoxide dismutase, SOD, SOD soluble, SOD1, SOD2, SODC, SODC_HUMAN, Superoxide dismutase 1 soluble, Superoxide Dismutase 1, Soluble, Superoxide dismutase Cu Zn, Superoxide dismutase cystolic, Superoxide dismutase [Cu-Zn], Superoxide Dismutase, Cystolic
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Superoxide Dismutase 1 Antibody (A84197) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Superoxide Dismutase 1 Antibody (A84197)
Superoxide Dismutase 1 expression in Mouse Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Superoxide Dismutase 1 Antibody (A84197) at 0.02µg/ml and detected by chemiluminescence.
Western Blot - Anti-Superoxide Dismutase 1 Antibody (A84197) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Superoxide Dismutase 1 Antibody (A84197)
Superoxide Dismutase 1 expression in NIH3T3 (A), HEK293 (B), HepG2 (C), and MCF7 (D) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Superoxide Dismutase 1 Antibody (A84197) at 0.01µg/ml and detected by chemiluminescence.
Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A84197) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A84197)
Superoxide Dismutase 1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Superoxide Dismutase 1 Antibody (A84197) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A84197) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A84197)
Superoxide Dismutase 1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Superoxide Dismutase 1 Antibody (A84197) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
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