Anti-SOCS2 Antibody [ARC1470] (A307926)

Name: Anti-SOCS2 Antibody [ARC1470]
Brand: Antibodies.com
SKU: A307926
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1470] antibody to SOCS2 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-SOCS2 Antibody [ARC1470]

Description

Rabbit monoclonal [ARC1470] antibody to SOCS2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-418 of human SOCS2 (O14508).

Sequence

MTLRCLEPSGNGGEGTRSQWGTAGSAEEPSPQAARLAKALRELGQTGWYWGSMTVNEAKEKLKEAPEGTFLIRDSSHSDYLLTISVKTSAGPTNLRIEYQDGKFRLDSIICVKSKLKQFDSVVHLIDYYVQMCKDKRTGPEAPRNGTVHLYLTKPLYTSAPSLQHLCRLTINKCTGAIWGLPLPTRLKDYLEEYKFQV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1470

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CIS 2, CIS-2, CIS2, Cish 2, Cish2, Cytokine inducible SH2 protein 2, Cytokine-inducible SH2 protein 2, SOCS 2, SOCS-2, SOCS2_HUMAN, SSI 2, SSI-2, SSI2, STAT induced STAT inhibitor 2, STAT-induced STAT inhibitor 2, STATI 2, STATI2, Suppressor of cytokine signaling 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-SOCS2 Antibody [ARC1470] (A307926)

Western blot analysis of extracts of K-562 cells, using Anti-SOCS2 Antibody [ARC1470] (A307926) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-SOCS2 Antibody [ARC1470] (A307926)

Western blot analysis of extracts of various cell lines, using Anti-SOCS2 Antibody [ARC1470] (A307926) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 3: Immunohistochemistry - Anti-SOCS2 Antibody [ARC1470] (A307926)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-SOCS2 Antibody [ARC1470] (A307926) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-SOCS2 Antibody [ARC1470] (A307926)

Immunofluorescence analysis of NIH-3T3 cells using Anti-SOCS2 Antibody [ARC1470] (A307926) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).