Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BOP, CD8 beta opposite, CD8b opposite, Histone lysine N methyltransferase SMYD1, KMT3D, SET and MYND domain containing 1, SET and MYND domain-containing protein 1, SMYD1_HUMAN, Zinc finger MYND domain containing 18, ZMYND18, ZMYND22
Figure 1: Western Blot - Anti-SMYD1 Antibody (A80437)
Western blot analysis of extracts of various cell lines, using Anti-SMYD1 Antibody (A80437) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-SMYD1 Antibody (A80437) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung using Anti-SMYD1 Antibody (A80437) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-SMYD1 Antibody (A80437) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
