Anti-SMURF 2 Antibody [ARC1897] (A308190)

Name: Anti-SMURF 2 Antibody [ARC1897]
Brand: Antibodies.com
SKU: A308190
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1897] antibody to SMURF 2 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-SMURF 2 Antibody [ARC1897]

Description

Rabbit monoclonal [ARC1897] antibody to SMURF 2.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 201-290 of human SMURF2 (Q9HAU4).

Sequence

PLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1897

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E3 ubiquitin-protein ligase SMURF2, EC 6.3.2., hSMURF2, MGC138150, Smad specific E3 ubiquitin ligase 2, SMAD specific E3 ubiquitin protein ligase 2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMUF2_HUMAN, Smurf2, Ubiquitin protein ligase SMURF2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Western blot analysis of extracts of various cell lines, using Anti-SMURF 2 Antibody [ARC1897] (A308190) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-SMURF 2 Antibody [ARC1897] (A308190) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Immunofluorescence analysis of NIH-3T3 cells using Anti-SMURF 2 Antibody [ARC1897] (A308190) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Immunoprecipitation analysis of 300µg extracts of mouse lung using 3µg of Anti-SMURF 2 Antibody [ARC1897] (A308190). This Western blot was performed on the immunoprecipitate using Anti-SMURF 2 Antibody [ARC1897] (A308190) at a dilution of 1:1000.

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