Anti-SMC1A (phospho Ser957) Antibody [ARC1606] (A306127)

Name: Anti-SMC1A (phospho Ser957) Antibody [ARC1606]
Brand: Antibodies.com
SKU: A306127
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC1606] antibody to SMC1A (phospho Ser957) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-SMC1A (phospho Ser957) Antibody [ARC1606]

Description

Rabbit monoclonal [ARC1606] antibody to SMC1A (phospho Ser957).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S957 of human SMC1 (Q14683).

Sequence

GSSQG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1606

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Chromosome segregation protein SmcB, DXS423E, KIAA0178, MGC138332, Sb1.8, Segregation of mitotic chromosomes 1, SMC protein 1A, SMC-1-alpha, SMC-1A, SMC1, SMC1 (structural maintenance of chromosomes 1 yeast) like 1, SMC1 structural maintenance of chromosomes 1 like 1, SMC1alpha, SMC1A_HUMAN, SMC1L1, SMCB, Structural maintenance of chromosomes 1A, Structural maintenance of chromosomes protein 1A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-SMC1A (phospho Ser957) Antibody [ARC1606] (A306127)

Western blot analysis of extracts of 293T cells, using Anti-SMC1A (phospho Ser957) Antibody [ARC1606] (A306127) at 1:1,000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-SMC1A (phospho Ser957) Antibody [ARC1606] (A306127)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-SMC1A (phospho Ser957) Antibody [ARC1606] (A306127) at 1:1,000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 3: Immunohistochemistry - Anti-SMC1A (phospho Ser957) Antibody [ARC1606] (A306127)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-SMC1A (phospho Ser957) Antibody [ARC1606] (A306127) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SMC1A (phospho Ser957) Antibody [ARC1606] (A306127)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-SMC1A (phospho Ser957) Antibody [ARC1606] (A306127) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.