Anti-SMC1A Antibody (A295225)

Name: Anti-SMC1A Antibody
Brand: Antibodies.com
SKU: A295225
Price: 425.0 USD
Availability: InStock

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$425

Rabbit polyclonal antibody to SMC1A for WB, IP and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-SMC1A Antibody

Description

Rabbit polyclonal antibody to SMC1A.

Applications

WB, IP, IHC

Dilutions

IHC: 1:2,000-1:10,000, IP: 2-10 µg/1 mg lysate, WB: 1:2,000-1:10,000

Reactivity

Human, Mouse

Immunogen

Synthetic peptide within amino acids 1175 and the C-terminus of human SMC1A (NP_006297. 2).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

General Notes

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonyms

Chromosome segregation protein SmcB, DXS423E, KIAA0178, MGC138332, Sb1.8, Segregation of mitotic chromosomes 1, SMC protein 1A, SMC-1-alpha, SMC-1A, SMC1, SMC1 (structural maintenance of chromosomes 1 yeast) like 1, SMC1 structural maintenance of chromosomes 1 like 1, SMC1alpha, SMC1A_HUMAN, SMC1L1, SMCB, Structural maintenance of chromosomes 1A, Structural maintenance of chromosomes protein 1A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunohistochemistry - Anti-SMC1A Antibody (A295225)

Sample: FFPE section of mouse colon carcinoma CT26. Antibody: Anti-SMC1A Antibody (A295225) was used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB.

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Figure 2: Immunohistochemistry - Anti-SMC1A Antibody (A295225)

Sample: FFPE section of human ovarian carcinoma. Antibody: Anti-SMC1A Antibody (A295225) was used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB.

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Figure 3: Immunoprecipitation - Anti-SMC1A Antibody (A295225)

Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Anti-SMC1A Antibody (A295225) was used for IP at 6 µg per reaction. SMC1A was also immunoprecipitated with a rabbit Anti-SMC1A recombinant monoclonal antibody. For blotting immunoprecipitated SMC1A, the Anti-SMC1A recombinant monoclonal antibody was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Figure 4: Western Blot - Anti-SMC1A Antibody (A295225)

Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Anti-SMC1A Antibody (A295225) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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