Anti-SMARCA6 Antibody (A15003)

Name: Anti-SMARCA6 Antibody
Brand: Antibodies.com
SKU: A15003
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to SMARCA6 for WB and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-SMARCA6 Antibody

Description

Rabbit polyclonal antibody to SMARCA6.

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human Lsh/HELLS (NP_060533.2).

Sequence

VYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FLJ10339, Helicase lymphoid specific, Hells, HELLS_HUMAN, LSH, Lymphoid specific helicase, Lymphoid-specific helicase, Nbla10143, PASG, Proliferation associated SNF2 like protein, Proliferation-associated SNF2-like protein, SWI/SNF2 related matrix associated actin dependent regulator of chromatin subfamily A member 6, SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-SMARCA6 Antibody (A15003)

Western blot analysis of extracts of various cell lines, using Anti-SMARCA6 Antibody (A15003) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-SMARCA6 Antibody (A15003)

Western blot analysis of extracts from normal (control) and Lsh/HELLS knockout (KO) HeLa cells, using Anti-SMARCA6 Antibody (A15003) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 3: Western Blot - Anti-SMARCA6 Antibody (A15003)

Immunoprecipitation analysis of 200µg extracts of 293T cells using 1µg of Anti-SMARCA6 Antibody (A15003). This Western blot was performed on the immunoprecipitate using Anti-SMARCA6 Antibody (A15003) at a dilution of 1:1000.

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