Anti-SMAD9 Antibody (A82495)

Name: Anti-SMAD9 Antibody
Brand: Antibodies.com
SKU: A82495
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to SMAD9 for ELISA, WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-SMAD9 Antibody

Description

Goat polyclonal antibody to SMAD9.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:64,000, WB: 0.3-1.0 µg/ml, IHC: 5µg/ml

Reactivity

Human

Cross Reactivity

This antibody is not expected to cross react with SMAD1 and SMAD5.

Immunogen

Synthetic peptide corresponding to the internal region of human SMAD9.

Sequence

C-HSEPLMPHNATYPD

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

50 kDa

Predicted MW

48.6kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

MAD homolog 9, Madh6, Mothers against decapentaplegic, Mothers against decapentaplegic homolog 9, Mothers against DPP homolog 9, SMAD 9, SMAD family member 9, SMAD, mothers against DPP homolog 9 (Drosophila), SMAD8A, SMAD8B, SMAD9_HUMAN

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-SMAD9 Antibody (A82495)

SMAD9 expression in Human Skin lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SMAD9 Antibody (A82495) at 0.3µg/ml and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-SMAD9 Antibody (A82495)

SMAD9 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-SMAD9 Antibody (A82495) at 5µg/ml and revealed with alkaline phosphatase (AP).

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