Western Blot - Anti-MADH7 / SMAD7 Antibody (A8636)
Western blot analysis of extracts of SW480 cells, using Anti-MADH7 / SMAD7 Antibody (A8636) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.