Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Name: Anti-SMAD5 (phospho Ser463 + Ser465) Antibody
Brand: Antibodies.com
SKU: A308813
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to SMAD5 (phospho Ser463 + Ser465) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-SMAD5 (phospho Ser463 + Ser465) Antibody

Description

Rabbit polyclonal antibody to SMAD5 (phospho Ser463 + Ser465).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S463 & S465 of human Phospho-Smad5-S463/S465 (NP_005894.3).

Sequence

SSVS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, SMAD5_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Western blot analysis of extracts of NIH/3T3, using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at 1:600 dilution. NIH/3T3 cells were treated by BMP4 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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