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Anti-SMAD5 Antibody [ARC55344] (A309477)

Western Blot - Anti-SMAD5 Antibody [ARC55344] (A309477) - Antibodies.com
Knockout Validated
$470
Product Datasheet

Rabbit monoclonal [ARC55344] antibody to SMAD5 for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-SMAD5 Antibody [ARC55344]
Description
Rabbit monoclonal [ARC55344] antibody to SMAD5.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Rat
Immunogen
Recombinant protein of human Smad5.
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC55344
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, SMAD5_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-SMAD5 Antibody [ARC55344] (A309477) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-SMAD5 Antibody [ARC55344] (A309477)
Western blot analysis of extracts from wild type(WT) and Smad5 knockout (KO) 293T(KO) cells, using Anti-SMAD5 Antibody [ARC55344] (A309477) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Publishing research using Anti-SMAD5 Antibody [ARC55344] (A309477)? Please let us know so that we can list the citation on this page.

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