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Smad4 Cell Based ELISA Kit (A103046)

Western Blot - Smad4 Cell Based ELISA Kit (CB5641) - Antibodies.com Protocol Diagram - Smad4 Cell Based ELISA Kit (CB5641) - Antibodies.com Protocol Summary - Smad4 Cell Based ELISA Kit (CB5641) - Antibodies.com Western Blot - Control Antibody - Smad4 Cell Based ELISA Kit (CB5641) - Antibodies.com
$550
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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Smad4 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This Smad4 Cell Based ELISA Kit allows for the detection of Smad4 and the effects that certain stimulation conditions have on Smad4 expression in different cell lines. Qualitative determination of Smad4 concentration is achieved by an indirect ELISA format. In essence, the Smad4 is captured by Anti-Smad4 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Smad4 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
(Small) Mothers Against Decapentaplegic, Deleted in Pancreatic Carcinoma, Deleted in Pancreatic Carcinoma 4, Deleted in pancreatic carcinoma locus 4, Deletion target in pancreatic carcinoma 4, DPC 4, DPC4, hSMAD4, JIP, MAD homolog 4, MAD mothers against decapentaplegic Drosophila homolog 4, MAD mothers against decapentaplegic homolog 4, MADH 4, MADH4, Med, Medea, Mothers against decapentaplegic homolog 4, Mothers against decapentaplegic, Drosophila, homolog of, 4, Mothers against DPP homolog 4, MYHRS, OTTHUMP00000163548, SMA- and MAD-related protein 4, SMAD 4, SMAD family member 4, SMAD mothers against DPP homolog 4, SMAD4_HUMAN
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Smad4 Cell Based ELISA Kit (CB5641) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Smad4 Cell Based ELISA Kit (A103046)
Western blot analysis of the lysates from HT-29 cells using Anti-Smad4 antibody.
Protocol Diagram - Smad4 Cell Based ELISA Kit (CB5641) - Antibodies.comEnlarge Image
Figure 2: Smad4 Cell Based ELISA Kit (A103046)
A diagram showing the protocol for Smad4 Cell Based ELISA Kit.
Protocol Summary - Smad4 Cell Based ELISA Kit (CB5641) - Antibodies.comEnlarge Image
Figure 3: Smad4 Cell Based ELISA Kit (A103046)
A summary of the protocol for Smad4 Cell Based ELISA Kit.
Western Blot - Control Antibody - Smad4 Cell Based ELISA Kit (CB5641) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Smad4 Cell Based ELISA Kit (A103046)
The mouse monoclonal antibody to GAPDH is used as a positive control inSmad4 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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