Anti-Smad4 Antibody [ARC5009-06] (A308139)

Name: Anti-Smad4 Antibody [ARC5009-06]
Brand: Antibodies.com
SKU: A308139
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC5009-06] antibody to Smad4 for WB, IHC, IP and ChIP.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-Smad4 Antibody [ARC5009-06]

Description

Rabbit monoclonal [ARC5009-06] antibody to Smad4.

Applications

WB, IHC, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000, CUT&Tag: 1⁶ cells/1µg

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 320-552 of human Smad4 (NP_005350.1).

Sequence

PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC5009-06

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

(Small) Mothers Against Decapentaplegic, Deleted in Pancreatic Carcinoma, Deleted in Pancreatic Carcinoma 4, Deleted in pancreatic carcinoma locus 4, Deletion target in pancreatic carcinoma 4, DPC 4, DPC4, hSMAD4, JIP, MAD homolog 4, MAD mothers against decapentaplegic Drosophila homolog 4, MAD mothers against decapentaplegic homolog 4, MADH 4, MADH4, Med, Medea, Mothers against decapentaplegic homolog 4, Mothers against decapentaplegic, Drosophila, homolog of, 4, Mothers against DPP homolog 4, MYHRS, OTTHUMP00000163548, SMA- and MAD-related protein 4, SMAD 4, SMAD family member 4, SMAD mothers against DPP homolog 4, SMAD4_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

Enlarge Image

Figure 1: Anti-Smad4 Antibody [ARC5009-06] (A308139)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1µg of Anti-Smad4 Antibody [ARC5009-06] (A308139), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of Smad4 in representative gene loci (JUNB), as shown in figure.

Enlarge Image

Figure 2: Western Blot - Anti-Smad4 Antibody [ARC5009-06] (A308139)

Western blot analysis of extracts of various cell lines, using Anti-Smad4 Antibody [ARC5009-06] (A308139) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Enlarge Image

Figure 3: Western Blot - Anti-Smad4 Antibody [ARC5009-06] (A308139)

Enlarge Image

Figure 4: Immunohistochemistry - Anti-Smad4 Antibody [ARC5009-06] (A308139)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Smad4 Antibody [ARC5009-06] (A308139) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.