Anti-Smad4 Antibody (A97217)

Name: Anti-Smad4 Antibody
Brand: Antibodies.com
SKU: A97217
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to Smad4 for WB and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Smad4 Antibody

Description

Rabbit polyclonal antibody to Smad4.

Applications

WB, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:10000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Smad4 (amino acids 21-70).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.

Molecular Weight

60kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

(Small) Mothers Against Decapentaplegic, Deleted in Pancreatic Carcinoma, Deleted in Pancreatic Carcinoma 4, Deleted in pancreatic carcinoma locus 4, Deletion target in pancreatic carcinoma 4, DPC 4, DPC4, hSMAD4, JIP, MAD homolog 4, MAD mothers against decapentaplegic Drosophila homolog 4, MAD mothers against decapentaplegic homolog 4, MADH 4, MADH4, Med, Medea, Mothers against decapentaplegic homolog 4, Mothers against decapentaplegic, Drosophila, homolog of, 4, Mothers against DPP homolog 4, MYHRS, OTTHUMP00000163548, SMA- and MAD-related protein 4, SMAD 4, SMAD family member 4, SMAD mothers against DPP homolog 4, SMAD4_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Smad4 Antibody (A97217)

Western blot analysis of lysates from HeLa, COS7, HepG and HUVEC cells using Anti-Smad4 Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-Smad4 Antibody (A97217)

Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using Anti-Smad4 Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.

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Figure 3: Immunohistochemistry - Anti-Smad4 Antibody (A97217)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Anti-Smad4 Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.

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Figure 4: Immunofluorescence - Anti-Smad4 Antibody (A97217)

Immunofluorescence analysis of mouse kidney tissue using Anti-Smad4 Antibody (red) at 1:200 (4°C overnight). Cy3 labelled secondary antibody was used at 1:300 (RT 50min). Panel A: Target. Panel B: DAPI. Panel C: Merge.