Anti-Smad3 Antibody (A97219)

Name: Anti-Smad3 Antibody
Brand: Antibodies.com
SKU: A97219
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to Smad3 for WB and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Smad3 Antibody

Description

Rabbit polyclonal antibody to Smad3.

Applications

WB, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:10000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Smad3 (amino acids 145-194).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.

Molecular Weight

48kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Smad3 Antibody (A97219)

Western blot analysis of lysates from HT-29 cells using Anti-Smad3 Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Western Blot - Anti-Smad3 Antibody (A97219)

Western blot analysis of various cells using Anti-Smad3 Antibody.

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Figure 3: Western Blot - Anti-Smad3 Antibody (A97219)

Western blot analysis of HT29 cells using Anti-Smad3 Antibody.

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Figure 4: Immunohistochemistry - Anti-Smad3 Antibody (A97219)

Immunohistochemical analysis of paraffin-embedded human uterus tissue using Anti-Smad3 Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.