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Anti-Smad3 Antibody (A95779)

Western Blot - Anti-Smad3 Antibody (B0031) - Antibodies.com Immunohistochemistry - Anti-Smad3 Antibody (B0031) - Antibodies.com Western Blot - Anti-Smad3 Antibody (B0031) - Antibodies.com
$185
Product Datasheet

Rabbit polyclonal antibody to Smad3 for WB, IHC and ELISA.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Smad3 Antibody
Description
Rabbit polyclonal antibody to Smad3.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:20000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Smad3 (amino acids 376-425).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.
Molecular Weight
48kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Smad3 Antibody (B0031) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Smad3 Antibody (A95779)
Western blot analysis of lysates from 293 cells, treated with UV 5' using Anti-Smad3 Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-Smad3 Antibody (B0031) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Smad3 Antibody (A95779)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-Smad3 Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-Smad3 Antibody (B0031) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-Smad3 Antibody (A95779)
Western blot analysis of various cells using Anti-Smad3 Antibody.
Publishing research using Anti-Smad3 Antibody (A95779)? Please let us know so that we can list the citation on this page.

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