Anti-Smad3 Antibody (A92961)

Name: Anti-Smad3 Antibody
Brand: Antibodies.com
SKU: A92961
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Smad3 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Smad3 Antibody

Description

Rabbit polyclonal antibody to Smad3.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 190-221 of human Smad3 (NP_005893.1).

Sequence

ETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

hMAD-3, hSMAD3, JV15-2, MAD homolog 3, Mad3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Smad3 Antibody (A92961)

Western blot analysis of extracts of various cell lines, using Anti-Smad3 Antibody (A92961) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunofluorescence - Anti-Smad3 Antibody (A92961)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Smad3 Antibody (A92961) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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