Anti-Smad2 (phospho Thr220) Antibody (A93815)

Name: Anti-Smad2 (phospho Thr220) Antibody
Brand: Antibodies.com
SKU: A93815
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to Smad2 (phospho Thr220) for IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Smad2 (phospho Thr220) Antibody

Description

Rabbit polyclonal antibody to Smad2 (phospho Thr220).

Specificity

This antibody detects endogenous levels of Smad2 only when phosphorylated at Thr220.

Applications

IF, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:20000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Smad2 around the phosphorylation site of Thr220 (amino acids 186-235).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

52kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hMAD 2, hMAD 3, hSMAD2, hSMAD3, Mad related protein 2, MADH2, MADH3, MADR2, Mothers against DPP homolog 2, Mothers against DPP homolog 3, Sma and Mad related protein 2, SMA and MAD related protein 3, SMAD 2, SMAD 3, SMAD family member 2, SMAD family member 3, smad2+3, smad2/3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Immunofluorescence - Anti-Smad2 (phospho Thr220) Antibody (A93815)

Immunofluorescence analysis of HeLa cells using Anti-Smad2 (phospho Thr220) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-Smad2 (phospho Thr220) Antibody (A93815)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-Smad2 (phospho Thr220) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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