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Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026)

Western Blot - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.com Immunohistochemistry - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.com Immunohistochemistry - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.com Immunohistochemistry - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.com Immunohistochemistry - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.com Immunofluorescence - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.com Immunofluorescence - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.com +1
This product is discontinued

Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody
Description
Rabbit polyclonal antibody to Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425).
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around S465 & S467 of human Phospho-Smad2-S465/467 + Smad3-S423/425SMAD2 (NP_005892.1).
Sequence
SSMS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (8)

Western Blot - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026)

Western blot analysis of C2C12, using Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) at 1:800 dilution. C2C12 cells were treated by TGF-beta (10 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-Smad2 (phospho Ser465 + Ser467) + Smad3 (phospho Ser423 + Ser425) Antibody (A11026) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.