Rabbit polyclonal antibody to Smad2 (Phospho-Ser465)
Specificity
The antibody detects endogenous levels of Smad2 only when phosphorylated at serine 465.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 465(C-S-S(p)-M-S) derived from Human Smad2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2
Western blot analysis of extracts from K562 cells using Smad2 (Phospho-Ser465) Antibody #11838.The lane on the right is treated with the antigen-specific peptide.
Publishing research using Anti-Smad2 (Phospho-Ser465) Antibody (A50977)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-Smad2 (Phospho-Ser465) Antibody (A50977)
![Western Blot - Anti-Smad2 Antibody [ARC0343] (A307856) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307856_1.jpg?profile=product_alternative)
![Western Blot - Anti-Smad2 Antibody [ARC57635] (A309474) - Antibodies.com](https://cdn.antibodies.com/image/catalog/309/A309474_1.jpg?profile=product_alternative)
![Western Blot - Anti-Smad2 (phospho Ser250) Antibody [ARC1552] (A305752) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305752_1.jpg?profile=product_alternative)
![Western Blot - Anti-Smad2 (phospho Ser465 + Ser467) Antibody [ARC56317] (A308802) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308802_1.jpg?profile=product_alternative)
![Western Blot - Anti-Smad2 (phospho Ser245 + Ser250 + Ser255) Antibody [ARC53851] (A308749) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308749_1.jpg?profile=product_alternative)
