Anti-Smad1 Antibody (A309166)

Name: Anti-Smad1 Antibody
Brand: Antibodies.com
SKU: A309166
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to Smad1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-Smad1 Antibody

Description

Rabbit polyclonal antibody to Smad1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human Smad1 (NP_005891.1).

Sequence

WKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BSP-1, BSP1, HsMAD1, JV4-1, JV41, MAD homolog 1, MAD mothers against decapentaplegic homolog 1, Mad related protein 1, Mad-related protein 1, MADH1, MADR1, Mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMA- AND MAD-RELATED PROTEIN 1, SMAD 1, SMAD family member 1, SMAD mothers against DPP homolog 1, SMAD1_HUMAN, TGF beta signaling protein 1, Transforming growth factor-beta-signaling protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Smad1 Antibody (A309166)

Western blot analysis of extracts of various cell lines, using Anti-Smad1 Antibody (A309166) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Smad1 Antibody (A309166)

Western blot analysis of extracts from wild type (WT) and Smad1 knockout (KO) HeLa cells, using Anti-Smad1 Antibody (A309166) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-Smad1 Antibody (A309166)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Smad1 Antibody (A309166) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Smad1 Antibody (A309166)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Smad1 Antibody (A309166) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.