Anti-SIRT5 Antibody [ARC59763] (A309471)

Name: Anti-SIRT5 Antibody [ARC59763]
Brand: Antibodies.com
SKU: A309471
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC59763] antibody to SIRT5 for WB and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-SIRT5 Antibody [ARC59763]

Description

Rabbit monoclonal [ARC59763] antibody to SIRT5.

Applications

WB, IP

Dilutions

WB: 1:1,000-1:5,000, IP: 1:1,000-1:5,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human SIRT5.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59763

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

NAD dependent deacetylase sirtuin 5, NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial, NAD dependent protein deacylase sirtuin 5 mitochondrial, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, Silent mating type information regulation 2 S.cerevisiae homolog 5, Sir2 like 5, SIR2-like protein 5, SIR2L5, SIR5_HUMAN, Sirtuin 5, Sirtuin type 5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-SIRT5 Antibody [ARC59763] (A309471)

Western blot analysis of 293F, using Anti-SIRT5 Antibody [ARC59763] (A309471) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-SIRT5 Antibody [ARC59763] (A309471)

Western blot analysis of various lysates, using Anti-SIRT5 Antibody [ARC59763] (A309471) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-SIRT5 Antibody [ARC59763] (A309471)

Immunoprecipitation analysis of 600µg extracts of mouse heart cells using 3µg of Anti-SIRT5 Antibody [ARC59763] (A309471). This Western blot was performed on the immunoprecipitate using Anti-SIRT5 Antibody [ARC59763] (A309471) at a dilution of 1:2000.

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