Anti-SIRT5 Antibody (A14975)

Name: Anti-SIRT5 Antibody
Brand: Antibodies.com
SKU: A14975
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to SIRT5 for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-SIRT5 Antibody

Description

Rabbit polyclonal antibody to SIRT5.

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human SIRT5 (NP_036373.1).

Sequence

MRPLQIVPSRLISQLYCGLKPPASTRNQICLKMARPSSSMADFRKFFAKAKHIVIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRAIAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEALACHENETVS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

NAD dependent deacetylase sirtuin 5, NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial, NAD dependent protein deacylase sirtuin 5 mitochondrial, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, Silent mating type information regulation 2 S.cerevisiae homolog 5, Sir2 like 5, SIR2-like protein 5, SIR2L5, SIR5_HUMAN, Sirtuin 5, Sirtuin type 5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-SIRT5 Antibody (A14975)

Western blot analysis of extracts from normal (control) and SIRT5 knockout (KO) 293T cells, using Anti-SIRT5 Antibody (A14975) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-SIRT5 Antibody (A14975)

Western blot analysis of extracts of various cell lines, using Anti-SIRT5 Antibody (A14975) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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