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Anti-SIRT2 Antibody (A12592)

Western Blot - Anti-SIRT2 Antibody (A12592) - Antibodies.com Immunohistochemistry - Anti-SIRT2 Antibody (A12592) - Antibodies.com Immunohistochemistry - Anti-SIRT2 Antibody (A12592) - Antibodies.com Immunohistochemistry - Anti-SIRT2 Antibody (A12592) - Antibodies.com Immunofluorescence - Anti-SIRT2 Antibody (A12592) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to SIRT2 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-SIRT2 Antibody
Description
Rabbit polyclonal antibody to SIRT2.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 288-389 of human SIRT2 (NP_036369.2).
Sequence
EKAGQSDPFLGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQSGAGVPNPSTSASPKKSPPPAKDEARTTEREKPQ
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
39 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
FLJ35621, FLJ37491, NAD dependent deacetylase sirtuin 2, NAD dependent protein deacetylase sirtuin 2, NAD-dependent deacetylase sirtuin-2, NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, Silencing information regulator 2 like, Silent information regulator 2, SIR2, SIR2 like protein 2, Sir2 related protein type 2, SIR2, S. cerevisiae, homolog-loke 2, SIR2-like protein 2, SIR2L, SIR2L2, SIRT2_HUMAN, Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae), Sirtuin 2, Sirtuin type 2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-SIRT2 Antibody (A12592) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-SIRT2 Antibody (A12592)
Western blot analysis of various lysates, using Anti-SIRT2 Antibody (A12592) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry - Anti-SIRT2 Antibody (A12592) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-SIRT2 Antibody (A12592)
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-SIRT2 Antibody (A12592) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-SIRT2 Antibody (A12592) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-SIRT2 Antibody (A12592)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-SIRT2 Antibody (A12592) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-SIRT2 Antibody (A12592) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-SIRT2 Antibody (A12592)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SIRT2 Antibody (A12592) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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