Anti-SIRT1 Antibody [PCRP-SIRT1-1E11] (A277616)

Mouse monoclonal [PCRP-SIRT1-1E11] antibody to SIRT1 for ELISA, IP, Flow Cytometry, IF and WB.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-SIRT1 Antibody [PCRP-SIRT1-1E11]
Description
Mouse monoclonal [PCRP-SIRT1-1E11] antibody to SIRT1.
Specificity
The silent information regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent matingtype loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
Applications
ELISA, IP, Flow Cytometry, IF, WB
Dilutions
IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant full-length human SIRT1 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
PCRP-SIRT1-1E11
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
120 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-SIRT1 Antibody [PCRP-SIRT1-1E11] - BSA and Azide free (A278204).
Synonyms
75SirT1, hSIR2, hSIRT1, HST2, S. cerevisiae, homolog of, NAD dependent deacetylase sirtuin 1, NAD dependent protein deacetylase sirtuin 1, NAD-dependent deacetylase sirtuin-1, OTTHUMP00000198111, OTTHUMP00000198112, Regulatory protein SIR2 homolog 1, SIR1_HUMAN, SIR2, SIR2 like 1, SIR2 like protein 1, SIR2, S.cerevisiae, homolog-like 1, SIR2-like protein 1, SIR2ALPHA, SIR2L1, SIRT1_HUMAN, SirtT1 75 kDa fragment, Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), Sirtuin 1, sirtuin 1 ((silent mating type information regulation 2 homolog) 1, Sirtuin type 1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-SIRT1 Antibody [PCRP-SIRT1-1E11] (A277616)? Please let us know so that we can list the citation on this page.

Alternative products to Anti-SIRT1 Antibody [PCRP-SIRT1-1E11] (A277616)

Top