Anti-SIRP alpha Antibody [ED9] (FITC) (A280339)

$420

Mouse monoclonal [ED9] antibody to SIRP alpha (FITC) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-SIRP alpha Antibody [ED9] (FITC)
Description
Mouse monoclonal [ED9] antibody to SIRP alpha (FITC).
Specificity
This antibody recognises rat Tyrosine-protein phosphatase non-receptor type substrate 1, also known as CD172a, Signal-regulatory protein alpha-1, SIRPa -1, SHP substrate 1, Macrophage membrane protein MFP150 or Macrophage fusion receptor. CD172a is a 509 amino acid ~56 kDa single pass type 1 transmembrane glycoprotein expressed selectively by myeloid cells and by neurons (UniProt: P97710). Mouse anti Rat CD172a antibody, clone ED9 has been reported to bind to an alternative epitpe to another anti CD172 antibody, clone OX-41 and has been reported to block the interaction of CD172a - CD47.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood.
Reactivity
Rat
Immunogen
Spleen cell homogenate.
Host
Mouse
Clonality
Monoclonal
Clone ID
ED9
Isotype
IgG1
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Rat CD172a antibody, clone ED9 recognizes rat Tyrosine-protein phosphatase non-receptor type substrate 1, also known as CD172a, Signal-regulatory protein alpha-1, SIRPa -1, SHP substrate 1, Macrophage membrane protein MFP150 or Macrophage fusion receptor. CD172a is a 509 amino acid ~56 kDa single pass type 1 transmembrane glycoprotein expressed selectively by myeloid cells and by neurons (UniProt: P97710). Mouse anti Rat CD172a antibody, clone ED9 has been reported to bind to an alternative epitpe to another anti CD172 antibody, clone OX-41 (Adams et al. 1998) and has been reported to block the interaction of CD172a - CD47 (de Vries et al. 2002).
Synonyms
Bit, Brain Ig like molecule with tyrosine based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, Brain immunoglobulin like molecule with tyrosine based activation motifs, CD172 antigen like family member A, CD172 antigen-like family member A, CD172a, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MYD 1, Myd 1 antigen, MyD-1 antigen, p84, Protein tyrosine phosphatase non receptor type substrate 1, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SHPS1_HUMAN, Signal regulatory protein alpha, Signal regulatory protein alpha 2, Signal regulatory protein alpha 3, Signal regulatory protein alpha type 1, Signal regulatory protein alpha type 2, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, SIRP, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3, SIRPA, SIRPalpha, SIRPalpha1, SIRPalpha2, SIRPalpha3, Tyrosine phosphatase SHP substrate 1, Tyrosine protein phosphatase non receptor type substrate 1, Tyrosine-protein phosphatase non-receptor type substrate 1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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